Сверхкритические флюиды. Теория и практика
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2014, N3, pp. 04-12

D.Yu. Zalepugin, V.V. Zaitzev, N.A. Tilkunova, I.V. Chernyshova, I.I. Selezneva, Yu.A. Nikonova, M.I. Vlasov

Xenogenic bone matrix processed with supercritical carbon dioxide as potential osteoplastic material

Multistage environmentally benign method of xenogenic bone matrix processing in sub- and supercritical media is developed. This method provides with a fast matrix deli pidation in supercritical carbon dioxide, deproteinization by treatment with 3% hydrogen peroxide solution followed by washing in subcritical water for removal of polar substances. The final treatment with supercritical carbon dioxide serves to remove residual non-polar components. A method to control the porosity of xenogenic bone matrix on several stages of purification is suggested. Preliminary data indicate that cell test system based on the allogenic osteoid cell line Th-1 can be used for evaluation of primary adhesion and cell proliferation on the surface of purified bone matrix.

Key words: supercritical extraction, porosity, carbon dioxide, xenogenic matrix, osteoplastic materials

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