Key words: salbutamol, enantiomers, supercritical fluid chromatography, polysaccharide-based chiral stationary phases
Three chiral chromatography stationary phases based on amylose and cellulose were
tested for the supercritical fluid chromatography separation of antiasthmatic drug
salbutamol enantiomers. A cellulose tris-3-chloro-4-methylphenylcarbamate stationary
phase provided the highest selectivity for this separation. The optimization of mobile
phase composition allowed us to maximize the selectivity coefficient and to minimize
the peak asymmetry factors. A successful salbutamol enantiomer separation is achieved
at moderate modifier fraction (10–15% vol.) in the mobile phase on this column.
Amine additives are necessary to suppress peak tailing. Among three tested amines
isopropylamine gives the most symmetrical peaks along with the highest enantioselectivity.
doi:10.1134/S1990793118070059