M.O. Kostenko, K.B. Ustinovich, O.I. Pokrovskiy, O.O. Parenago, N.G. Bazarnova, V.V. Lunin
Effect of Mobile Phase Composition on Selectivity in Supercritical Fluid Chromatography in Salbutamol Enantiomer Separation
Three chiral chromatography stationary phases based on amylose and cellulose were
tested for the supercritical fluid chromatography separation of antiasthmatic drug
salbutamol enantiomers. A cellulose tris-3-chloro-4-methylphenylcarbamate stationary
phase provided the highest selectivity for this separation. The optimization of mobile
phase composition allowed us to maximize the selectivity coefficient and to minimize
the peak asymmetry factors. A successful salbutamol enantiomer separation is achieved
at moderate modifier fraction (10–15% vol.) in the mobile phase on this column.
Amine additives are necessary to suppress peak tailing. Among three tested amines
isopropylamine gives the most symmetrical peaks along with the highest enantioselectivity.
Key words: salbutamol, enantiomers, supercritical fluid chromatography, polysaccharide-based chiral stationary phases