2018, №3

сс. 67-76

Comparison of Hold-up Time Determination Methods in Supercritical Fluid Chromatography

2018, №3

сс. 67-76

Цитировать

M.O. Kostenko, O.I. Pokrovskiy, O.O. Parenago, V.V. Lunin

Key words: hold-up time, supercritical fluid chromatography, system peak, nonretentive compound, static method

Three most common methods for measuring chromatographic hold-up time in
supercritical fluid chromatography are tested. It is shown that at low concentrations of
polar cosolvents in the mobile phase, the use of the simplest «system peak» method
leads to highly inaccurate results. With an increase in the percentage of co-solvent to
10—15 %, this method can be used for rough estimation of hold-up time values,
especially for non-polar stationary phases. The use of nitric oxide(I) as a «nonretentive»
hold-up time marker, on the contrary, is well suited only in the case of pure
carbon dioxide as a mobile phase. The static method theoretically can give very precise
values of hold-up time, but it requires the knowledge of mobile phase velocity profile
along the whole chromatograph tract.

doi:10.1134/S1990793119070145