Key words: supercritical and water-alcoholic extracts, Ascidia, Halocynthia aurantium, bio-test, sea urchins, embryo, exogastrulation, Infusoria
Strongylocentrotus intermedius is studied. It is showen that water-alcohol extract has no remarkable effect onto gametes, process fertilization, development of embryo and larvae of sea urchin. If the SC extract is added during the gamete fusion, the fertilization and cell-division of blastomers are not affected, but the gastrulation becomes abnormal — the exogastrulas are formed. Moreover, during the cultivation of embryo in a cultural medium with SC extracts, the growth of Infusoria is depressed. It is likely that the origin of differences in the biological effects of SC and water-alcohol extracts ought to look for in peptide or secondary metabolite fractions, since no distinct differences in li pid fractions are detected.