Key words: Hypogymnia physodes, supercritical fluid extraction, low molecular weight phenols, flavonoids, atranorine, antibacterial activity
The process of supercritical fluid extraction of the thallus of the lichen species Hypogymnia physodes with carbon dioxide in the presence of acetone as a co-solvent has been studied. An assessment of extraction conditions (temperature, pressure and duration) on the yields of extract, phenolic compounds, including low molecular weight phenols, atranorine and flavonoids was carried out. It has been shown that the main amount of extractives (up to 90 %) is extracted within 40 minutes of extraction. The extract yield reaches 156.5 mg/g, with a content of phenolic compounds up to 87.3 %. The extracts obtained have high antiradical activity (up to 225 rmol Trolox-equiv/g). A positive correlation has been established between the antiradical activity of extracts and the content of phenolic compounds in them, including atranorine. Antibacterial activity of the extracts was revealed against gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli, Proteus mirabilis) strains of microorganisms.
doi:10.34984/SCFTP.2024.19.1.006