Key words: avermectin, yield, Streptomyces avermitilis, supercritical fluid extraction, carbon dioxide, rotatable uniform plan, optimization.
The process of supercritical fluid extraction of avermectin from the bacterial biomass of Streptomyces avermitilis with carbon dioxide was optimized by the method of a full factorial experiment using a second-order rotatable uniform plan. When planning, the main levels chosen were a pressure of 25 MPa, a temperature of 60°C, and an extraction duration of 100 min. The analysis of the regression equation made it possible to establish the optimal process parameters: 30 MPa, 85°C, and 133 min. In the control experiment, an extract was obtained with a yield of 13 %, containing 41 % avermectin. The yield of avermectin was 5.36 % of absolutely dry biomass, which exceeds the extraction yields obtained using organic solvents.
doi:10.1134/S1990793124701033