Key words: supercritical drying, aerogel, chitosan, protein, pharmaceutical compositions, drug delivery systems
Protein (egg white) and chitosan aerogel microparticles characterized by low density, developed morphology and high specific surface area, high porosity, large volume and small pore diameter, were obtained. Using the laser diffraction method, particle size distribution curves were obtained, from which the average number and aerodynamic diameters were calculated. The aerodynamic diameter of all obtained biopolymer aerogels lies in the range required for targeted delivery of active pharmaceutical ingredients (APIs) to the olfactory region of the nasal cavity. Experimental studies have been carried out on the introduction of API «melatonin» into the pores of biopolymer aerogels at the stages of solvent replacement and supercritical adsorption. Mass fractions of API in the resulting pharmaceutical compositions «biopolymer aerogel — API» were determined by high-performance liquid chromatography. X-ray powder diffraction showed that API in pharmaceutical compositions is present mainly in the amorphous state. The results of in vivo experimental studies showed that in the case of intranasal administration of both «biopolymer aerogel — API» compositions, the maximum concentration of API in brainтtissues is reached after 30 minutes. This proves that the resulting biopolymer aerogels based on protein (egg white) and chitosan are promising for use as API carrier matrices in the development of nasal drug delivery systems.
doi:10.34984/SCFTP.2023.18.1.001
Cite
ГОСТ
7.0.100-2018
Biopolymer aerogels as nasal drug delivery systems / – Текст : непосредственный // Сверхкритические Флюиды: Теория и Практика.. – 2023. – Т. 1. – № 1. – С. 2-23. - DOI: 10.34984/SCFTP.2023.18.1.001
TRANSLIT
Biopolimernye aerogeli kak nazalnye sistemy dostavki lekarstv / – Tekst : neposredstvennyj // Sverhkriticheskie Flyuidy: Teoriya i Praktika.. – 2023. – Т. 1. – № 1. – S. 2-23. - DOI: 10.34984/SCFTP.2023.18.1.001
En
Biopolymer aerogels as nasal drug delivery systems. Sverhkriticheskie Flyuidy: Teoriya i Praktika, 1, 2-23 (2023). https://doi.org/10.34984/SCFTP.2023.18.1.001
Key words: sub- and supercritical fluid extraction, ethanol, tinder fungus, fomes fomentarius, chitin-containing complex.
Chitin-containing complexes were obtained from the fruiting body of the tinder fungus Fomes fomentarius using sub- and supercritical fluid extraction with ethanol with a yield of 57—88 % and a chitin content of up to 18.7 %. The influence of the duration, temperature, and pressure of extraction on the yield and properties of the resulting chitin containing complexes has been evaluated. Chitin-containing complexes were characterized by elemental and functional (by IR spectroscopy) composition. The high sorption capacity of the obtained complexes in relation to the basic — methylene blue (up to 312 mg/g) and acid — congo red (up to 170 mg/g) dyes is shown. Sorption activity relative to both basic and acid dyes indicates the polyampholytic nature of the resulting complexes-biosorbents.
doi:10.34984/SCFTP.2023.18.1.002
Cite
ГОСТ
7.0.100-2018
Obtaining chitin-containing complexes from the fruit body of the <i>Fomes fomentarius</i> fungus using sub- and supercritical extraction methods / – Текст : непосредственный // Сверхкритические Флюиды: Теория и Практика.. – 2023. – Т. 1. – № 1. – С. 24-37. - DOI: 10.34984/SCFTP.2023.18.1.002
TRANSLIT
Poluchenie hitinsoderzhashchih kompleksov iz plodovogo tela griba <i>Fomes fomentarius</i> metodami sub- i sverhkriticheskoj ekstrakcii / – Tekst : neposredstvennyj // Sverhkriticheskie Flyuidy: Teoriya i Praktika.. – 2023. – Т. 1. – № 1. – S. 24-37. - DOI: 10.34984/SCFTP.2023.18.1.002
En
Obtaining chitin-containing complexes from the fruit body of the <i>Fomes fomentarius</i> fungus using sub- and supercritical extraction methods. Sverhkriticheskie Flyuidy: Teoriya i Praktika, 1, 24-37 (2023). https://doi.org/10.34984/SCFTP.2023.18.1.002
Key words: supercritical carbon dioxide, resorcinol-formaldehyde aerogels, ferrocene
An efficient method for the synthesis of hybrid resorcinol-formaldehyde aerogels with ferrocene subunit fragments embedded in their polymer structure has been developed. In the process of gel formation preceding the production of aerogels, dioxane was used for the first time as the main reaction solvent. The conversion of gels into aerogels was carried out using SC-CO2). Aerogel samples were characterized in detail by Mossbauer spectroscopy, nitrogen adsorption, and atomic absorption spectroscopy, and the density of materials was determined. The iron content in the resulting aerogels varies in the range of 1.5—11 % by weight of iron (or 5.3—35 % by weight of ferrocene units). Metallocene subunits are present in aerogels in two valence-oxidative states: ferrocene and ferrocenium.
doi:10.34984/SCFTP.2023.18.1.003
Cite
ГОСТ
7.0.100-2018
Ferrocene-modified resorcinol-formaldehyde aerogels / – Текст : непосредственный // Сверхкритические Флюиды: Теория и Практика.. – 2023. – Т. 1. – № 1. – С. 38-51. - DOI: 10.34984/SCFTP.2023.18.1.003
TRANSLIT
Modificirovannye ferrocenom rezorcin-formal'degidnye aerogeli / – Tekst : neposredstvennyj // Sverhkriticheskie Flyuidy: Teoriya i Praktika.. – 2023. – Т. 1. – № 1. – S. 38-51. - DOI: 10.34984/SCFTP.2023.18.1.003
En
Ferrocene-modified resorcinol-formaldehyde aerogels. Sverhkriticheskie Flyuidy: Teoriya i Praktika, 1, 38-51 (2023). https://doi.org/10.34984/SCFTP.2023.18.1.003
The extraction of Japanese sophora buds (Sophora japonica) (BS) was carried out using a subcritical water medium (SBW) within a temperature of 120—220°C, as well as by the traditional water-ethanol method. Evaluation of the polyphenolic composition of extracts and the activity of acetylcholinesterase (AChE) inhibition for products isolated by various methods showed that the SBW extract obtained at a temperature of 220 °C demonstrates the highest values in terms of the sum of polyphenols and the sum of flavonoids, both in comparison with the extract obtained by the traditional method and with SBW extracts, isolated at lower temperatures. At the same time, among the studied products, SBW extract (temperature 220 °C) shows the highest activity in inhibiting AHE, which indicates the determining role of the sum of polyphenols in the composition of BS extracts.
doi:10.34984/SCFTP.2023.18.1.004
Cite
ГОСТ
7.0.100-2018
Evaluation of the dependence of the polyphenolic composition and the activity of acetylcholinesterase inhibition of extracts of japanese sophora buds (<i>Sophora japonica</i>) on the extraction method / – Текст : непосредственный // Сверхкритические Флюиды: Теория и Практика.. – 2023. – Т. 1. – № 1. – С. 52-61. - DOI: 10.34984/SCFTP.2023.18.1.004
TRANSLIT
/ – Tekst : neposredstvennyj // Sverhkriticheskie Flyuidy: Teoriya i Praktika.. – 2023. – Т. 1. – № 1. – S. 52-61. - DOI: 10.34984/SCFTP.2023.18.1.004
En
Evaluation of the dependence of the polyphenolic composition and the activity of acetylcholinesterase inhibition of extracts of japanese sophora buds (<i>Sophora japonica</i>) on the extraction method. Sverhkriticheskie Flyuidy: Teoriya i Praktika, 1, 52-61 (2023). https://doi.org/10.34984/SCFTP.2023.18.1.004
Key words: supercritical carbon dioxide, subcritical carbon dioxide, poly-D,L-lactide, polymethylmethacrylate, electron spin resonance spectroscopy, 2,2,6,6-tetramethyl-piperidine-1-oxyl, spin probe, diffusion coefficient, spin-spin exchange interactions, distribution constant, supercritical fluid chromatography
The rotational and translational coefficients of the TEMPONE spin probe mobility were studied by ESR spectroscopy in the PLA and PMMA-based polymers plasticized with supercritical as well as subcritical carbon dioxide. Translational diffusion coefficients of TEMPONE in the PMMA-SC-CO2, system are 3 times inferior in comparison to PLA-SC-CO2 and the corresponding values are of 10-14 and 10-11 m2/s order of magnitude. The rotation activation energy of a value 22±5 kJ/mole was determined for the TEMPONE rotation in PLA-SC-CO2. It was found out that desorption of the radical exceeds TEMPONE diffusion into bulk PLA under subcritical conditions during the experimental period. Spectra of TEMPONE in PLA-SUB-CO2 turned out to be a superposition of two signals: a narrow one, which is similar to that of TEMPONE in PLA-SC-CO2, and a broad isotropic one caused by the TEMPONE paramagnetic particles accumulation in a polymer volume proximate to the surface. As local concentration decreases with time, the rotational anisotropy is exposed in the latter signal. The study gives an outlook on the SC-CO2 use as promising media to the polymer impregnation primarily taking into consideration both it’s and solutes’ higher mass diffusivity.
doi:10.34984/SCFTP.2023.18.1.005
Cite
ГОСТ
7.0.100-2018
The diffusion of tempone spin probe in poly-D,L-lactide and polymethylmethacrylate plasticized with sub- and supercritical carbon dioxide / – Текст : непосредственный // Сверхкритические Флюиды: Теория и Практика.. – 2023. – Т. 1. – № 1. – С. 63-86. - DOI: 10.34984/SCFTP.2023.18.1.005
TRANSLIT
Diffuziya spinovogo zonda TEMPON v plastificirovannyh sub- i sverhkriticheskim CO<sub>2 </sub>polimetilmetakrilate i poli-D, L-laktide / – Tekst : neposredstvennyj // Sverhkriticheskie Flyuidy: Teoriya i Praktika.. – 2023. – Т. 1. – № 1. – S. 63-86. - DOI: 10.34984/SCFTP.2023.18.1.005
En
The diffusion of tempone spin probe in poly-D,L-lactide and polymethylmethacrylate plasticized with sub- and supercritical carbon dioxide. Sverhkriticheskie Flyuidy: Teoriya i Praktika, 1, 63-86 (2023). https://doi.org/10.34984/SCFTP.2023.18.1.005