2009, №3

сс. 16-24

Influence of Supercritical and Water-alcohol Extracts from Halocynthia aurantium onto Gametes, Zygotes and Embryo of Sea Urchin

2009, №3

сс. 16-24

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A.L. Drozdov, T.I. Chizhova, V.M. Chudnovskiy, V.I. Yusupov, O.I. Pokrovskiy, O.O. Parenago, N.G. Busarova, S.V. Isai, V.N. Bagratashvili

Key words: supercritical and water-alcoholic extracts, Ascidia, Halocynthia aurantium, bio-test, sea urchins, embryo, exogastrulation, Infusoria

Strongylocentrotus intermedius is studied. It is showen that water-alcohol  extract has no
remarkable effect onto gametes, process  fertilization, development of embryo and
larvae of sea urchin. If the SC extract is  added during the gamete fusion, the fertilization
and cell-division of blastomers are not  affected, but the gastrulation becomes abnormal —  
the exogastrulas are formed. Moreover, during  the cultivation of embryo in a cultural
medium with SC extracts, the growth of  Infusoria is depressed. It is likely that the
origin of differences in the biological  effects of SC and water-alcohol extracts ought to
look for in peptide or secondary metabolite  fractions, since no distinct differences in
li pid fractions are detected.

doi:10.1134/S199079310908003X