Key words: supercritical CO2 extraction, micellar fungi, lipids, methyl esters of fatty acids, biodiesel
In the course of fermentation of oleogenic fungi at 27—28 °C during 4—5 days li pids (triglycerides of fatty acids) are produced with a 60 % yield in terms of dry biomass. Triglycerides are extracted by supercritical carbon dioxide in a flow regime at 80— 100 °C and 50 MPa. Methyl esters of fatty acids are synthesized by two-step transesterification of triglycerides with a 98 % yield. Using a combined chromatography/ mass-spectrometry method it is demonstrated that the fatty-acid composition of Cunninghamella echinulata fungi is closest to that of rapeseed oil.
doi:10.1134/S1990793114080028