2013, №4

сс. 46-52

Production of methyl esters of fatty acids — bases for biodiesel fuel — from micellar fungi lipids extracted by supercritical carbon dioxide

2013, №4

сс. 46-52

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V.I. Bogdan, A.E. Koklin, V.G. Krasovsky, V.V. Lunin, Ya.E. Sergeeva, A.A. Ivashechkin, E.P. Feofilova

Key words: supercritical CO2 extraction, micellar fungi, lipids, methyl esters of fatty acids, biodiesel

In the course of fermentation of oleogenic fungi at 27—28 °C during 4—5 days li pids (triglycerides of fatty acids) are produced with a 60 % yield in terms of dry biomass. Triglycerides are extracted by supercritical carbon dioxide in a flow regime at 80— 100 °C and 50 MPa. Methyl esters of fatty acids are synthesized by two-step transesterification of triglycerides with a 98 % yield. Using a combined chromatography/ mass-spectrometry method it is demonstrated that the fatty-acid composition of Cunninghamella echinulata fungi is closest to that of rapeseed oil.

doi:10.1134/S1990793114080028