Key words: supercritical extraction, garlic, carbon dioxide, thiosulfinates, high performance liquid chromatography (HPLC)
For the first time a detailed investigation of destruction of thiosulfinates from garlic
supercritical extract in solvents of different polarity (water, ethyl alcohol, acetonitrile,
dimethylsulfoxide, methylene chloride and hexane) is performed. Qualitative and
quantitative analysis of thiosulfinate destruction products is carried out. A new HPLC
analytical procedure is developed that offers a possibility to determine both initial
thiosulfinates, as well as their sulfur-containing destruction products. As a result, a way
of directing the thiosulfinates destruction process to the formation of desired substances
(ajoenes, dithiines, trisulfides, etc.) is suggested. The obtained results may be used for
studying medico-biological applications of garlic products, and also for their
manufacturing, storage and analysis.
doi:10.1134/S1990793115070143