Key words: porcine cartilage, decellularization, surfactants, supercritical CO2, ethanol, histological studies, DNA, mesenchymal stromal cells
At obtaining tissue-specific scaffolds of dense tissues from decellularized porcine
articular cartilage it was found that full decellularization of tissue takes surfactant
treatment followed by supercritical fluides treatment. An introduction of ethanol as a
polarity modifier enables to increase significantly a completeness of cartilage
decellularization backgrounded by reduction of the destructive changes in the
extracellular matrix. Preservation of morphology and active proliferation of mesenchymal
stromal cells of human adi pose tissue in the presence of tissue-specific scaffold of
porcine cartilage evidence an opportunity of its use as a cell carrier for tissue
engineering of cartilage.
doi:10.1134/S1990793120080059