Key words: supercritical fluid chromatography, enantiomers, chiral separation, semipreparative separation methods, analytical separation methods, ibuprofen, ketoprofen
Highly efficient analytical methods for separating racemic mixtures of ibuprofen and ketoprofen with supercritical fluid chromatography (SFC) have been developed using a chiral stationary phase based on amylose and supercritical carbon dioxide in combination with a co-solvent as a mobile phase. Optimum values of the resolution and selectivity for each pair of enantiomers have been achieved both in the isocratic and gradient modes. It has been revealed that the separation efficiency significantly depends on the co-solvent content in the mobile phase. The possibility of scaling the developed methods for semipreparative analysis has been assessed.